Signalling

Part:BBa_K511301:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)


Notch-Gal4-VP16 Juxtacrine Signaling Receptor MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 258
    Illegal PstI site found at 663
    Illegal PstI site found at 993
    Illegal PstI site found at 1224
    Illegal PstI site found at 1321
    Illegal PstI site found at 2511
    Illegal PstI site found at 4389
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 258
    Illegal PstI site found at 663
    Illegal PstI site found at 993
    Illegal PstI site found at 1224
    Illegal PstI site found at 1321
    Illegal PstI site found at 2511
    Illegal PstI site found at 4389
    Illegal NotI site found at 5224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2933
    Illegal XhoI site found at 5750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 258
    Illegal PstI site found at 663
    Illegal PstI site found at 993
    Illegal PstI site found at 1224
    Illegal PstI site found at 1321
    Illegal PstI site found at 2511
    Illegal PstI site found at 4389
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 258
    Illegal PstI site found at 663
    Illegal PstI site found at 993
    Illegal PstI site found at 1224
    Illegal PstI site found at 1321
    Illegal PstI site found at 2511
    Illegal PstI site found at 4389
    Illegal NgoMIV site found at 524
    Illegal NgoMIV site found at 2636
    Illegal NgoMIV site found at 2692
    Illegal NgoMIV site found at 2735
    Illegal NgoMIV site found at 3520
    Illegal NgoMIV site found at 4537
    Illegal NgoMIV site found at 4714
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5669
    Illegal BsaI.rc site found at 85
    Illegal BsaI.rc site found at 1801
    Illegal BsaI.rc site found at 2716
    Illegal BsaI.rc site found at 5173


Design Notes

Not applicable.


Source

This part is derived from the human Notch-1 receptor, the DNA-binding domain of the Gal4 transactivation protein from Saccharomyces cerevisiae, and the viral VP16 transactivation domain from the herpes simplex virus.


References